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By Tadayuki Imanaka (auth.)

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30 31 32 34 34 35 35 36 38 39 39 39 41 41 41 42 42 43 43 43 44 44 46 46 46 48 48 50 50 Recent new techniques such as genetic engineering and/or cell fusion have made it possible to manipulate genes and to make microorganisms intentionally have various desired properties. Using these useful techniques, wc have succeeded in the breeding of functional microorganisms which can produce large a m o u n t s of A T P or glutathione (a detoxifiTzation drug for liver). This review deals with the construction of microbial bioreactor or biocatalyst systems by the use of r e c D N A techniques and with the production of glutathione by genetically engineered cells.

1. Machinery of the bioreactor or biocatalyst system I Application of recDNA Techniques 31 colytic pathway 3~. Therefore, glucose was not used as an energy source until some amount of ATP was accumulated in the reaction mixture. At the beginning, ATP was produced by the use of glycogen present in cells, because glycogen phosphorylase, which was not inhibited by the high amount of phosphate, hydrolyzes glycogen gradually to produce glucose-6-phosphate (G6P). The G6P thus generated flowed into the glycolytic pathway and was then further metabolized to generate ATP.

30 31 32 34 34 35 35 36 38 39 39 39 41 41 41 42 42 43 43 43 44 44 46 46 46 48 48 50 50 Recent new techniques such as genetic engineering and/or cell fusion have made it possible to manipulate genes and to make microorganisms intentionally have various desired properties. Using these useful techniques, wc have succeeded in the breeding of functional microorganisms which can produce large a m o u n t s of A T P or glutathione (a detoxifiTzation drug for liver).

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