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Erlanson-Albertsson, C. (1980a), Bwchim. Bwphys. Acta, 61 7 , 371. Erlanson-Albertsson, C. , 117, 295. -P. J. , 50, 227. , and Hansson, B. (1979), Eur. J . , 99, 353. Flanagan, S. D. and Barondes, S. H. (1975),J. B i d . , 250, 1484. -A. (1974), Eur. J. , 46, 75. -R. (1977), Eur. J. , 74, 199. Johansson, G. (1976), Biochim. Biophys. Acta, 451, 517. -A. (1973), Eur. J . , 33, 379. LeCoffic, H. , Langrene, H. , and Pasquier, A. (1980),Analyt. ,107, 417. , and Philipsson, L. (1976), Biochemistry, 15, 5754.

Shanbhag, V. -A. (1973), J . Sfer. , 4 , 537. 24 PER-AKEALBERTSSON Silvetman, D. , and Tu, C. (1979),J. B i d . , 254, 2588. , Carstensen, H. and Albertsson, P. A. (1983), Scand. J . Clin. Lab. , in press. , Rajkowski, K. ,andCittanova, N. ,119,253. , Gordon, J . , and Pestka, S. (1976). Antimicrobial Agents and Chemotherapy, 665. Walter, H. and Sasakawa, S. (1971), Biochenrirtry, 10, 108. Winlund, C. C. and Chamberlin, M. J. (1970), Biochem. Biophys. Res. , 40, 43. METHODS OF BIOCHEMICAL ANALYSIS VOLUME 29 Gel Sieving Electrophoresis: A Description of Procedures and Analysis of Errors GEORGE JOHNSON, I.

Because the functions M o and K, = f (crosslinker) prove to be linear reciprocal functions, we can vary crosslinker concentration without destroying the linearity of (2) if the concentration is varied in a linear reciprocal fashion. A simple way to d o this is to dilute (all dilution functions are linear reciprocals). 184 g/lOO ml. By using progressively higher concentrations of monomer in the construction of a series of gels, the investigator is effectively diluting the crosslinker with increasing concentrations of monomer.

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