By Takuji Sasaki, Jianzhong Wu, Hiroshi Mizuno, Baltazar A. Antonio, Takashi Matsumoto (auth.), Prof. Dr. Hiro-Yuki Hirano, Prof. Dr. Yoshio Sano, Prof. Dr. Atsushi Hirai, Dr. Takuji Sasaki (eds.)
Rice (Oryza sativa) is without doubt one of the most crucial staple nutrition plants on the earth. Breeding efforts to enhance the agronomical caliber of rice were carried out, and stories on rice from the point of view of simple organic curiosity have additionally been performed. In 1991, a booklet entitled Rice (edited by way of Dr. Bajaj) used to be released because the 14th quantity within the sequence Biotechnology in Agriculture and Forestry (BAF), detailing rice learn actions at the moment, and focusing regularly on mobile and tissue tradition and genetic variability. experiences on rice have essentially complicated on account that then, whose results are pointed out lower than. this can be a sturdy cause to bring together a brand new quantity on rice. the location relating to rice study has markedly replaced within the final sixteen years. First, the genomic sequences of rice have been thoroughly decided through the overseas Rice Genome Sequencing undertaking in 2004. because the genome series of Arabidopsis thaliana have been decided in 2000, rice turned the second one species within the seed crops to have its genome good understood. moment, the expertise to rework rice by means of the Agrobacterium-mediated technique used to be constructed and is now demonstrated. In classical phytopathology, Poaceae (including rice) has now not been regarded as a bunch for Agrobacterium. this change approach is comparatively effortless and reproducible in comparison to standard transformation tools utilizing protoplasts, and is now regular in rice research.
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Extra info for Rice Biology in the Genomics Era
3 Sequencing-based Measurements of mRNA and Small RNA 33 cleavage and degradation of the target molecule or repression of translation. In addition, siRNAs play a role in directing DNA methylation in the nuclear genome and the production of heterochromatin. In both cases, the end result is a negative regulation of gene expression. Because of the high specificity of these RNAdirected events, the use of both siRNAs, and more recently the development of artificial miRNAs, has been and continues to be an important tool for applications in plant biotechnology (Baulcombe 2004; Schwab et al.
Genetics 169:891–906 Tarchini R, Biddle P, Wineland R, et al. (2000) The complete sequence of 340 kb of DNA around the rice Adh1-Adh2 region reveals interrupted colinearity with maize chromosome 4. Plant Cell 12:381–391 The Rice Annotation Project (2007) Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana. Genome Res 17:175–185 The Rice Chromosome 3 Sequencing Consortium (2005) Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species.
The RAP-DB provides users with the IRGSP genome assembly and the RAP annotation as well as a number of functions to retrieve information about the rice genome. The annotation can be viewed through two different browsers, GBrowse and G-integra (Stein et al. 2002; Imanishi et al. 2004). The contents of the RAP-DB are listed in Table 4. GBrowse displays the rice genes and their functional descriptions. The rice genes are depicted in three tracks on the basis of their supporting evidence; transcripts with full-length cDNAs, predicted genes with ESTs, and genes predicted by pure ab initio methods.